Identification of various Biomphalaria alexandrina strains collected from five Egyptian governorates using RAPD and species-specific PCR techniques

Identification of various Biomphalaria alexandrina strains collected from five Egyptian governorates using RAPD and species-specific PCR techniques

The first generation of Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr El-Sheikh, Ismailia and Damietta) were subjected to species-specific PCR assays and the results showed that snails collected from the field were B. alexandrina, and there was no evidence for the presence of B. glabrata. The snails were subjected also to RAPD-PCR technique. The results showe...

The relationship between genetic variability and the susceptibility of Biomphalaria alexandrina snails to Schistosoma mansoni infection.

In the present study, Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr El-Sheikh, Ismailia and Damietta), as well as reference control Biomphalaria alexandrina snails from the Schistosome Biological Supply Center (SBSC) (Theodor Bilharz Research Institute, Egypt), were subjected to species-specific polymerase chain reaction (PCR) assays to identify the collected...

Genetic Similarity Among the Three Egyptian Water Buffalo Flocks Using RAPD-PCR and PCR-RFLP Techniques

Genetic similarity and polymorphisms among the three Egyptian water buffalo flocks (Beheiry, Minoufy and Saidy) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Ten random primers were used to amplify DNA fragments in these three flocks. RAPD patterns with a level of polymorphism were detected among flocks. The resul...

Isolation and Identification of Infectious Laryngotrachitis Virus from Commercial Flocks of Iran Using Various Techniques

Metacercaria Identification Using Hat-rapd Pcr Method

The infection dynamics of metacercariae were assessed in cyprinoid fish (cyprinoids) from Chiang Mai and Sakon Nakhon Provinces, Thailand, during October 2008 to September 2009. The samples were collected during 3 seasons from rivers and local markets. Metacercarial infection was determined by acidpepsin digestion and confirmed using HAT-RAPD PCR method. Thirteen and 16 species of cyprinoids we...